A mutant ofMycoplasma mycoidessubsp.mycoideslacking the H2O2-producing enzyme l-α-glycerophosphate oxidase

Cloning and characterization of cDNA encoding glyoxal oxidase, a H2O2-producing enzyme from the lignin-degrading basidiomycete Phanerochaete chrysosporium.

Glyoxal oxidase is produced by ligninolytic cultures of the white-rot fungus Phanerochaete chrysosporium and is a source of the extracellular H2O2 that is required by ligninolytic peroxidases. We report here the cloning and characterization of glx-1c cDNA, which encodes glyoxal oxidase. The deduced mature protein has 537 amino acids, a molecular size of 57 kDa, and a pI of 5.1. Five potential N...

L-alpha-Glycerophosphate dehydrogenase, a component of an oxidase system in Trypanosoma rhodesiense.

Structure and proposed mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae.

UNLABELLED The formation of H2 O2 by the FAD-dependent L-α-glycerophosphate oxidase (GlpO) is important for the pathogenesis of Streptococcus pneumoniae and Mycoplasma pneumoniae. The structurally known GlpO from Streptococcus sp. (SspGlpO) is similar to the pneumococcal protein (SpGlpO) and provides a guide for drug design against that target. However, M. pneumoniae GlpO (MpGlpO), having < 20%...

Investigation of the Catalytic Mechanism of Mycoplasma Pneumoniae L-alpha-glycerophosphate Oxidase: Mutation of the Proposed Catalytic Base

Vaginal Protection by H2O2-Producing Lactobacilli

Background: Peroxide-producing lactobacilli provide protection from infection for the female reproductive tract. However, in vitro studies demonstrated that H2O2-produced by Lactobacillus is not the cause of inhibition of pathogens. It is not exactly known how H2O2producing lactobacilli are involved in the protection of the vaginal environment. Objectives: This study aimed to evaluate the impor...